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Peptid-baserede identifikation af funktionskravene motiver og deres bindingspartnere
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Peptide-based Identification of Functional Motifs and their Binding Partners

Peptid-baserede identifikation af funktionskravene motiver og deres bindingspartnere

12,296 Views

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14:28 min

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June 30, 2013

DOI:

10.3791/50362-v

DOI:
10.3791/50362-v

•

14:28 min
June 30, 2013

•

2 Views
, , , , , ,
1Department of Microbiology, Biochemistry, & Immunology,Morehouse School of Medicine, 2Institute for Systems Biology, 3Advanced Medical & Dental Institute,Universiti Sains Malaysia

Summary

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Teknikker til at dissekere mekanismerne bag udskillelsen af ​​HIV-1 Nef i exosomes beskrevet. Specifikke korte peptider afledt fra Nef og protein transfektion blev udnyttet til at bestemme strukturen, funktionen og bindingspartnere af Nef s Sekretion Ændring Region. Disse procedurer har generel relevans i mange mekanistiske undersøgelser.

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Cite this Article

Shelton, M. N., Huang, M. B., Ali, S., Johnson, K., Roth, W., Powell, M., Bond, V. Peptide-based Identification of Functional Motifs and their Binding Partners. J. Vis. Exp. (76), e50362, doi:10.3791/50362 (2013).

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