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Peptide-based Identification of Functional Motifs and their Binding Partners
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

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14:28 min

June 30, 2013

DOI:

14:28 min
June 30, 2013

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Summary

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Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.

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