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Immunology and Infection

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JoVE Journal Immunology and Infection
Peptide-based Identification of Functional Motifs and their Binding Partners
 

Peptide-based Identification of Functional Motifs and their Binding Partners

Article DOI: 10.3791/50362-v • 14:28 min • June 30th, 2013
June 30th, 2013 •
1,2, 1, 1,3, 1, 1, 1, 1
1Department of Microbiology, Biochemistry, & Immunology, Morehouse School of Medicine, 2Institute for Systems Biology, 3Advanced Medical & Dental Institute, Universiti Sains Malaysia

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Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.

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Peptide-based Identification Functional Motifs Binding Partners Short Peptides Full-length Proteins HIV-1 Nef Biological Function Competitive Inhibition Apoptosis Induction Apoptotic Motifs Secretion Modification Region (SMR) Exosomes Bait Protein Co-immunoprecipitation Experiments Cellular Proteins SMR Motif Protein Transfection Antibody Inhibition Mortalin ExNef Secretion Assay Inhibitor Of ExNef Secretion
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