Journal
/
Imunologia e Infecção
/
使用骨髓源性巨噬细胞巨噬细胞极化的调查
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
Please note that all translations are automatically generated. Click here for the English version.

使用骨髓源性巨噬细胞巨噬细胞极化的调查

DOI:
10.3791/50323-v

•

10:07 min

•

June 23, 2013

•

, , , ,
1Department of Animal Science,Texas A&M University, 2Department of Biology,Texas A&M University, 3Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University

Capítulos

  • 00:05Título
  • 01:36Isolation of Bone Marrow Cells
  • 03:23Induction BMDM Formation
  • 04:17BMDM Polarized Activation
  • 06:11Representative Analysis of BMDM
  • 09:19Conclusion

Summary

Tadução automática

Tadução automática

本文介绍了一种自适应容易容易<em>在体外</em>模型，探讨巨噬细胞极化。在存在下GM-CSF/M-CSF，从骨髓中的造血干/祖细胞定向分化成单核细胞，由M1或M2的刺激。激活状态可以跟踪的细胞表面抗原，基因表达和细胞信号转导通路的变化。

Tags

Macrophage PolarizationBone Marrow Derived MacrophagesGM-CSFM-CSFMonocytic DifferentiationM1 PolarizationM2 PolarizationCell Surface AntigensGene ExpressionCell Signaling Pathways

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

弓形虫囊肿壁形成激活骨髓衍生的巨噬细胞和Bradyzoite条件

小鼠骨髓来源的树突状细胞与跟踪研究的Qdot纳米晶的生成及标签

一个<em在体外</em>模型研究人类巨噬细胞分化和极化的异质性

骨髓衍生的巨噬细胞产

隔离和小鼠骨髓的静脉注射单核细胞衍生

偏光M1和M2骨髓来源的巨噬细胞的使用实时外流量分析代谢表征

鼠后肢长骨解剖和骨髓隔离

代和GM-CSF的鉴定源性肺泡状巨噬细胞和树突状细胞从小鼠骨髓

巨噬细胞集落刺激因子分化的人单核细胞衍生的巨噬细胞的培养

抗体药物对小鼠骨髓及腹腔巨噬细胞活化作用的评价

Read Article