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立体定向注射病毒载体的基因操作在小鼠脊髓条件
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Neurociência
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JoVE Journal Neurociência
Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord
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立体定向注射病毒载体的基因操作在小鼠脊髓条件

DOI:
10.3791/50313-v

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08:39 min

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March 18, 2013

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, , ,
1Département Nociception et Douleur, Institut des Neurosciences Cellulaires et Intégratives,Centre National de la Recherche Scientifique (CNRS), 2Departments of Anesthesiology and Pharmacology,Columbia University , 3Department of Anesthesiology,Niigata University Graduate School of Medical and Dental Sciences

Capítulos

  • 00:05Título
  • 01:27Preparation of Equipment and Virus Particle Suspension
  • 03:09Laminectomy
  • 04:57Injection
  • 06:21Wound Closure and Postoperative Care
  • 07:35Results: Transfection of Dorsal Horn Neurons in the Mouse Spinal Cord
  • 08:08Conclusion

Summary

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Tadução automática

病毒载体可以进行有针对性的基因操作。我们提出了一个条件性基因的表达或消融方法在小鼠脊髓，采用立体的病毒载体注射到脊髓背角，初级躯体感觉传入中枢神经系统的神经元之间的突触接触的一个突出的部位。

Tags

Gene ManipulationStereotaxic InjectionViral VectorMouse Spinal CordConditional Gene ExpressionGene SilencingDorsal HornLaminectomyAdeno-associated VirusReporter GeneEGFPTransfection

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