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Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord
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Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord
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08:39 min
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March 18, 2013
DOI:
1Département Nociception et Douleur, Institut des Neurosciences Cellulaires et Intégratives,Centre National de la Recherche Scientifique (CNRS), 2Departments of Anesthesiology and Pharmacology,Columbia University , 3Department of Anesthesiology,Niigata University Graduate School of Medical and Dental Sciences
Summary
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Viral vectors allow for targeted gene manipulation. We demonstrate a method for conditional gene expression or ablation in the mouse spinal cord, using stereotaxic injection of a viral vector into the dorsal horn, a prominent site of synaptic contact between primary somatosensory afferents and neurons of the central nervous system.
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Inquimbert, P., Moll, M., Kohno, T., Scholz, J. Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord. J. Vis. Exp. (73), e50313, doi:10.3791/50313 (2013).
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