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13:32 min
April 10, 2013
DOI:
10.3791/50308-v
我们描述了如何使用激光捕获显微切割(LCM),以获得丰富的海马神经元群体或单个神经元,随后采用实时定量PCR和/或整个基因组微阵列基因表达分析的损伤大鼠的大脑冰冻切片。
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Boone, D. R., Sell, S. L., Hellmich, H. L. Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury. J. Vis. Exp. (74), e50308, doi:10.3791/50308 (2013).
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