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鸡胚胎脊髓片培养协议
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Chicken Embryo Spinal Cord Slice Culture Protocol
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鸡胚胎脊髓片培养协议

DOI:
10.3791/50295-v

•

10:57 min

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March 25, 2013

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, ,
1Research Department of Cell and Developmental Biology,University College London

Capítulos

  • 00:05Título
  • 01:15Chick Embryo Preparation
  • 03:33Embryo Slice Preparation
  • 05:48Culturing Embryo Slices
  • 06:22Sectioning the Slices for Immunostaining
  • 07:49Results: Development of the Lateral Motor Column in Chicken Embryo Slices
  • 10:35Conclusion

Summary

Tadução automática

Tadução automática

切片文化促进胚胎发育的基因和药理学的扰动操作。然而，文化条件必须确保可以继续正常发展的片内减少环境。我们说明了一个协议，有利于脊髓发育正常进行至少24小时。

Tags

Keywords: Spinal Cord Slice CultureChicken EmbryoMotor NeuronsNeurodevelopmentNeurotrophic FactorsIn Vitro CultureVibratome SlicingAgarose Embedding

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