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Chicken Embryo Spinal Cord Slice Culture Protocol
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March 25, 2013
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Slice cultures facilitate the manipulation of embryo development by gene and pharmacological perturbations. However, culture conditions must ensure that normal development can proceed within the reduced environment of the slice. We illustrate a protocol that facilitates normal spinal cord development to proceed for at least 24 hr.
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Tubby, K. C., Norval, D., Price, S. R. Chicken Embryo Spinal Cord Slice Culture Protocol. J. Vis. Exp. (73), e50295, doi:10.3791/50295 (2013).
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