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Chicken Embryo Spinal Cord Slice Culture Protocol
Chapitres
- 00:05Titre
- 01:15Chick Embryo Preparation
- 03:33Embryo Slice Preparation
- 05:48Culturing Embryo Slices
- 06:22Sectioning the Slices for Immunostaining
- 07:49Results: Development of the Lateral Motor Column in Chicken Embryo Slices
- 10:35Conclusion
Slice cultures facilitate the manipulation of embryo development by gene and pharmacological perturbations. However, culture conditions must ensure that normal development can proceed within the reduced environment of the slice. We illustrate a protocol that facilitates normal spinal cord development to proceed for at least 24 hr.
