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长期的嗅觉适应性的分子读数<em> C.线虫</em

JoVE Journal
Neurociência

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JoVE Journal Neurociência

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

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长期的嗅觉适应性的分子读数<em> C.线虫</em

DOI:

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11:30 min

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December 22, 2012

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Chao He, Jin I. Lee, Noelle L’Etoile, Damien O’Halloran

¹Department of Biological Sciences and Institute for Neuroscience,George Washington University, ²Fred Hutchinson Cancer Research Center, ³Department of Cell and Tissue Biology,University of California San Francisco

Capítulos

00:05Título
01:57Cultivation and Maintenance of Animals for Nuclear Translocation Assays
03:26Long-term Odor Induced Nuclear Translocation Assays
05:44Scoring the Odor-induced Nuclear Translocation Event
08:54Results: Molecular Readout of Long-term Olfactory Adaptation in C. elegans
11:09Conclusion

Summary

Tadução automática

在这里，我们描述了一个长期的嗅觉适应性的分子读出<em>秀丽隐杆线虫</em>。蛋白激酶G，EGL-4，稳定的适应措施是必要的，在初级感觉神经元对AWC。在长时间的气味暴露EGL-4转位从细胞质到细胞核的AWC。

Tags

Molecular Readout Long-term Olfactory Adaptation C. Elegans Sensory Neurons Adaptation Response Olfactory Circuit Genetic Screen Mutants Volatile Odors Amphid Wing Cells Type C (AWC) Sensory Neurons Chemotaxis Behavioral Assay Short-term Olfactory Adaptation Long-term Olfactory Adaptation Protein Kinase G (PKG)EGL-4 Protein

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