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主要鼠Ⅱ型肺泡上皮细胞的功能及分子生物学研究的流式细胞仪分离
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
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主要鼠Ⅱ型肺泡上皮细胞的功能及分子生物学研究的流式细胞仪分离

DOI:
10.3791/4322-v

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14:48 min

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December 26, 2012

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, , , , ,
1Research Group Immune Regulation,Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology,Otto-von-Guericke University , 3Department of Experimental Immunology,Helmholtz Centre for Infection Research

Capítulos

  • 00:05Título
  • 01:53Preparation of the Mouse Lung
  • 04:02Enzymatic Digestion of the Lung Tissue
  • 05:40Preparation of the Lung Cell Suspension
  • 08:02Antibody Staining for Flow Cytometric Cell-sorting
  • 08:58Cell-sorting
  • 10:36Representative Results
  • 14:07Conclusion

Summary

Tadução automática

Tadução automática

我们描述了迅速隔离的主要鼠Ⅱ型肺泡上皮的细胞（AECII）流式细胞仪的负选择。这些AECII显示活力和纯度高，适用于范围广泛的功能和分子生物学研究，他们的作用，自身免疫性疾病或感染性疾病，如呼吸系统疾病。

Tags

Flow Cytometric IsolationPrimary Murine Type II Alveolar Epithelial CellsFunctional StudiesMolecular StudiesImmune RegulationCytokine ProductionAntigen-presenting CellsInfectionT-cell Mediated AutoimmunityClinical ContextsAirway Hyper-reactivityInfectionsCell LinesLarge Numbers Of CellsComplex ProcessesAutoimmune Inflammation

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