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Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
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JoVE Journal 免疫学と感染
Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

25,888 Views

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14:48 min

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December 26, 2012

DOI:

10.3791/4322-v

DOI:
10.3791/4322-v

•

14:48 min
December 26, 2012

•

23 Views
, , , , ,
1Research Group Immune Regulation,Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology,Otto-von-Guericke University , 3Department of Experimental Immunology,Helmholtz Centre for Infection Research

概要

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We describe the rapid isolation of primary murine type II alveolar epithelial cells (AECII) by flow cytometric negative selection. These AECII show high viability and purity and are suitable for a wide range of functional and molecular studies regarding their role in respiratory conditions such as autoimmune or infectious diseases.

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Flow Cytometric IsolationPrimary Murine Type II Alveolar Epithelial CellsFunctional StudiesMolecular StudiesImmune RegulationCytokine ProductionAntigen-presenting CellsInfectionT-cell Mediated AutoimmunityClinical ContextsAirway Hyper-reactivityInfectionsCell LinesLarge Numbers Of CellsComplex ProcessesAutoimmune Inflammation

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Cite this Article

Gereke, M., Autengruber, A., Gröbe, L., Jeron, A., Bruder, D., Stegemann-Koniszewski, S. Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies. J. Vis. Exp. (70), e4322, doi:10.3791/4322 (2012).

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