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Imunologia e Infecção
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Mass Spectrometric Analysis of Glycosphingolipid Antigens
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

16,317 Views

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13:09 min

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April 16, 2013

DOI:

10.3791/4224-v

DOI:
10.3791/4224-v

•

13:09 min
April 16, 2013

•

9 Views
, ,
1Undergraduate Program,Rice University , 2Proteomics Facility, Department of Pathology,University of Texas MD Anderson Cancer Center , 3Department of Melanoma Medical Oncology,University of Texas MD Anderson Cancer Center , 4University of Texas Graduate School of Biological Sciences at Houston

Summary

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A specific and sensitive method to gain insight into the expression profile of glycosphingolipid antigens in immune organs and cells is described. The method takes advantage of the ion trap mass spectrometry allowing step-wise fragmentation of glycosphingolipid molecules for structural analysis in comparison to chemically synthesized standards.

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Cite this Article

Yin, A. B., Hawke, D., Zhou, D. Mass Spectrometric Analysis of Glycosphingolipid Antigens. J. Vis. Exp. (74), e4224, doi:10.3791/4224 (2013).

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