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Mass Spectrometric Analysis of Glycosphingolipid Antigens
Chapters
- 00:05Title
- 01:47Extraction of Glycosphingolipids from Rat Leukemia Cells
- 02:50Separation of Neutral and Acidic Lipids
- 05:03Removal of Phospholipids from the Neutral Fraction
- 08:14Permethylation of Glycosphingolipids
- 10:04Results: Mass Spectra of Permethylated Glycosphingolipids
- 12:12Conclusion
A specific and sensitive method to gain insight into the expression profile of glycosphingolipid antigens in immune organs and cells is described. The method takes advantage of the ion trap mass spectrometry allowing step-wise fragmentation of glycosphingolipid molecules for structural analysis in comparison to chemically synthesized standards.
Tags
Mass Spectrometric AnalysisGlycosphingolipid AntigensGSL’sGlycoconjugate BiomacromoleculesStructural InformationEmbryonic DevelopmentSignal TransductionImmune Receptor RecognitionSugar MoietiesIsomersLipid MoietiesFatty Acyl Chain LengthUnsaturationHydroxylationCarbohydrate PortionCeramide PortionTri-hexosylceramidesGlobotriaosylceramide (Galα4Galβ4Glcβ1Cer)Isoglobotriaosylceramide (Galα3Galβ4Glcβ1Cer)Molecular MassesSugar LinkagesBiological FunctionsModification Of Ceramide PartAlpha-galactosylceramideCytokine Secretion ProfilesInvariant NKT CellsCancer ModelsAutoimmune DiseasesStructural Analysis Of IsomersImmune Organs And Cells
