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在GFP标记的下丘脑神经元的小鼠脑片成像钙反应
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Neurociência
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JoVE Journal Neurociência
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
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在GFP标记的下丘脑神经元的小鼠脑片成像钙反应

17,635 Views

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09:14 min

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August 24, 2012

DOI:

10.3791/4213-v

DOI:
10.3791/4213-v

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09:14 min
August 24, 2012

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2 Views
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1Department of Physiology, School of Medicine,University of Saarland, Homburg, Germany

Tags

ImagingCalcium ResponsesGFP-tagged NeuronsHypothalamic Mouse Brain SlicesEncoding Of InformationMolecular StepsNeurohormone Signal Transduction CascadesIntracellular Ca2+Synaptic SitesGreen Fluorescent Protein (GFP)Spatially And Temporally Stimulus-induced Ca2+ ResponsesNon-green Fluorescent Ca2+ Indicator DyeConfocal MicroscopyRatiometric Ca2+ Indicator Fura-2Ultraviolet (UV) Light

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Cite this Article

Schauer, C., Leinders-Zufall, T. Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices. J. Vis. Exp. (66), e4213, doi:10.3791/4213 (2012).

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