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Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
JoVE Journal
Medicina
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JoVE Journal Medicina
Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

DOI:
10.3791/4162-v

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08:47 min

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August 21, 2012

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, ,
1Laboratory for Orthopedic Research,Balgrist University Hospital, Zurich

Capítulos

  • 00:05Título
  • 01:12In-situ Lung Perfusion and Fixation
  • 03:30Visualizing lacZ-tagged Metastatic Tumor Cells in Entire Lung Lobes
  • 04:31Visualizing lacZ-tagged Metastatic Tumor Cells in Lung Cryosections
  • 05:48Metastases Analysis in Dunn/LM8 Osteosarcoma-bearing Mice
  • 08:00Conclusion

Summary

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Tadução automática

The novel protocol reported in the present study allows selective detection of lung metastases at single cell resolution in mice by combined in-situ lung perfusion and fixation and X-Gal staining of lacZ-tagged tumor cells.

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Improved VisualizationLung MetastasesSingle Cell ResolutionMiceIn-situ PerfusionX-Gal StainingLacZ-tagged Tumor CellsMicrometastasesRadiologic ImagingTherapeutic EradicationAnimal ModelsPesquisa do CâncerMonitoring/visualizationPreclinical StudiesClinical TrialsDetection TechniquesMicro-computer Tomography (micro-CT)Positron Emission Tomography (PET)Bioluminescence ImagingFluorescence ImagingOptimization TechniquesPrimary Tumor GrowthOsteosarcoma ModelsEx Vivo AnalysisQPCRFACSHistological Staining

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