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Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
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August 21, 2012
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The novel protocol reported in the present study allows selective detection of lung metastases at single cell resolution in mice by combined in-situ lung perfusion and fixation and X-Gal staining of lacZ-tagged tumor cells.
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Improved VisualizationLung MetastasesSingle Cell ResolutionMiceIn-situ PerfusionX-Gal StainingLacZ-tagged Tumor CellsMicrometastasesRadiologic ImagingTherapeutic EradicationAnimal ModelsKrebsforschungMonitoring/visualizationPreclinical StudiesClinical TrialsDetection TechniquesMicro-computer Tomography (micro-CT)Positron Emission Tomography (PET)Bioluminescence ImagingFluorescence ImagingOptimization TechniquesPrimary Tumor GrowthOsteosarcoma ModelsEx Vivo AnalysisQPCRFACSHistological Staining
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Arlt, M. J., Born, W., Fuchs, B. Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells. J. Vis. Exp. (66), e4162, doi:10.3791/4162 (2012).
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