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Automated Quantification of Synaptic Fluorescence in C. elegans
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August 10, 2012
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The abundance of neurotransmitter receptors clustered at synapses strongly influences synaptic strength. This method quantifies fluorescently-labeled neurotransmitter receptors in three dimensions with single-synapse resolution in C. elegans, allowing hundreds of synapses to be rapidly characterized within a single sample without distortions introduced by z-plane projection.
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Automated QuantificationSynaptic FluorescenceC. ElegansSynapse StrengthNeurotransmitter ReceptorsSynaptic SitesPostsynaptic MembraneReceptor TraffickingSynaptic PlasticityNeuromodulationFluorescence MicroscopySynaptically-localized Neurotransmitter Receptor AbundanceSynaptic DevelopmentSynaptic PlasticityCaenorhabditis ElegansNative SynapsesAutomated IdentificationThree Dimensions
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Cite this Article
Sturt, B. L., Bamber, B. A. Automated Quantification of Synaptic Fluorescence in C. elegans. J. Vis. Exp. (66), e4090, doi:10.3791/4090 (2012).
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