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Automated Quantification of Synaptic Fluorescence in C. elegans
Capitoli
- 00:05Titolo
- 01:29Preparation of Worms for Imaging
- 04:50Confocal Imaging
- 07:34Automated Identification and Analysis of Individual Synaptic Clusters
- 10:28Representative Results
- 11:58Conclusion
The abundance of neurotransmitter receptors clustered at synapses strongly influences synaptic strength. This method quantifies fluorescently-labeled neurotransmitter receptors in three dimensions with single-synapse resolution in C. elegans, allowing hundreds of synapses to be rapidly characterized within a single sample without distortions introduced by z-plane projection.
Tags
Automated QuantificationSynaptic FluorescenceC. ElegansSynapse StrengthNeurotransmitter ReceptorsSynaptic SitesPostsynaptic MembraneReceptor TraffickingSynaptic PlasticityNeuromodulationFluorescence MicroscopySynaptically-localized Neurotransmitter Receptor AbundanceSynaptic DevelopmentSynaptic PlasticityCaenorhabditis ElegansNative SynapsesAutomated IdentificationThree Dimensions
