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Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
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Biologia
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JoVE Journal Biologia
Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

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13:13 min

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December 03, 2012

DOI:

10.3791/3995-v

DOI:
10.3791/3995-v

•

13:13 min
December 03, 2012

•

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1Department of Biology, Howard Hughes Medical Institute,University of Utah

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Nano-fEMProtein LocalizationPhoto-activated Localization MicroscopyElectron MicroscopyFluorescence MicroscopyImmuno-electron MicroscopyCorrelative ApproachesFiducial MarkersPlastic EmbeddingDiffraction Limit Of LightNano-resolution Fluorescence Electron Microscopy

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Watanabe, S., Richards, J., Hollopeter, G., Hobson, R. J., Davis, W. M., Jorgensen, E. M. Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy. J. Vis. Exp. (70), e3995, doi:10.3791/3995 (2012).

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