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Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
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生物学
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JoVE Journal 生物学
Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

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13:13 min

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December 03, 2012

DOI:

10.3791/3995-v

DOI:
10.3791/3995-v

•

13:13 min
December 03, 2012

•

2 Views
, , , , ,
1Department of Biology, Howard Hughes Medical Institute,University of Utah

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We describe a method to localize fluorescently tagged proteins in electron micrographs. Fluorescence is first localized using photo-activated localization microscopy on ultrathin sections. These images are then aligned to electron micrographs of the same section.

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Cite this Article

Watanabe, S., Richards, J., Hollopeter, G., Hobson, R. J., Davis, W. M., Jorgensen, E. M. Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy. J. Vis. Exp. (70), e3995, doi:10.3791/3995 (2012).

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