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多光子显微镜的清除小鼠脑组织表达YFP
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JoVE Journal Neurociência
Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP
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多光子显微镜的清除小鼠脑组织表达YFP

DOI:
10.3791/3848-v

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10:03 min

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September 23, 2012

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, , ,
1Department of Biomedical Engineering,Yale University, 2Department of Biomedical Engineering,Louisiana Tech University

Capítulos

  • 00:00Título
  • 03:08Animal Perfusion and Whole Mouse Brain Clearing
  • 06:57Microscope Setup
  • 08:12Representative Results and Conclusion

Summary

Tadução automática

Tadução automática

整个小鼠脏器的多光子显微镜能够通过光学成像之前清零器官，但并非所有协议保留的荧光蛋白的荧光信号。使用光学乙醇脱水和苯甲醇苯甲酸苄酯结算的结算方法，显示高分辨率的多光子图像的整个小鼠脑组织表达YFP。

Tags

Multiphoton MicroscopyCleared Mouse BrainYFP ExpressionIntrinsic FluorescenceSecond Harmonic Generation (SHG)Optically ClearingMethanol DehydrationBenzyl Alcohol:benzyl Benzoate (BABB)Fluorescent SignalOptical Clearing ProtocolsEthanol Graded SeriesFluorescent ProteinsMultiphoton ImagingHigh-resolution ImagesNeurons

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