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Medición de actividad de factor V en el plasma humano usando un ensayo de coagulación de microplacas
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
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Medición de actividad de factor V en el plasma humano usando un ensayo de coagulación de microplacas

18,759 Views

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13:08 min

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September 09, 2012

DOI:

10.3791/3822-v

DOI:
10.3791/3822-v

•

13:08 min
September 09, 2012

•

7 Views
, ,
1Applied Bioscience Program, Faculty of Science,University of Ontario Institute of Technology , 2Nursing Program, Faculty of Health Sciences,University of Ontario Institute of Technology , 3Medical Laboratory Science Program, Faculty of Health Sciences,University of Ontario Institute of Technology

Summary

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Este estudio describe un ensayo en microplaca novela que mide la actividad FV coagulación durante la formación del coágulo de fibrina en el plasma humano que no se ha informado anteriormente. El método utiliza un lector de microplacas cinético para medir continuamente el cambio en la absorbancia a 405 nm durante la formación del coágulo de fibrina en plasma humano.

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Cite this Article

Tilley, D., Levit, I., Samis, J. A. Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay. J. Vis. Exp. (67), e3822, doi:10.3791/3822 (2012).

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