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测量人体血浆中的凝血因子V活性，用酶联凝固分析
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
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测量人体血浆中的凝血因子V活性，用酶联凝固分析

18,759 Views

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13:08 min

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September 09, 2012

DOI:

10.3791/3822-v

DOI:
10.3791/3822-v

•

13:08 min
September 09, 2012

•

7 Views
, ,
1Applied Bioscience Program, Faculty of Science,University of Ontario Institute of Technology , 2Nursing Program, Faculty of Health Sciences,University of Ontario Institute of Technology , 3Medical Laboratory Science Program, Faculty of Health Sciences,University of Ontario Institute of Technology

Summary

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本研究描述了一种新颖的微孔板法测量FV凝血活性在人血浆中的纤维蛋白凝块的形成还没有被以前的报告。该方法使用一个动能酶标仪人血浆中的纤维蛋白凝块的形成过程中，连续地测量在405nm处的吸光度变化。

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Cite this Article

Tilley, D., Levit, I., Samis, J. A. Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay. J. Vis. Exp. (67), e3822, doi:10.3791/3822 (2012).

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