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抛光和钢筋，颅骨变薄窗口，为长期的小鼠脑成像

JoVE Journal
Neurociência

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JoVE Journal Neurociência

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

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抛光和钢筋，颅骨变薄窗口，为长期的小鼠脑成像

DOI:

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09:49 min

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March 07, 2012

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Andy Y. Shih, Celine Mateo, Patrick J. Drew³, Philbert S. Tsai, David Kleinfeld⁴

¹Department of Physics,University of California, San Diego, ²Department of Engineering Science and Mechanics,Pennsylvania State University , ³Department of Neurosurgery,Pennsylvania State University , ⁴Section of Neurobiology,University of California, San Diego

Capítulos

00:05Título
01:40Surgery Preparation and Animal Mounting
04:02The Generation of a Polished and Reinforced Thinned-skull Window
07:14Imaging Preparation
08:20Representative Results for Long-term Imaging and optical Manipulation of the Mouse Cortex
09:30Conclusion

Summary

Tadução automática

我们提出了一个方法，形成一个横跨毫米，是没有大脑的炎症个月稳定在小鼠颅骨的影像窗口。这种方法非常适合于使用双光子显微镜的血液流通，细胞动力学，细胞/血管结构的纵向研究。

Tags

Polished And Reinforced Thinned-skull Window Long-term Imaging Mouse Brain In Vivo Imaging Cortical Function Optical Access Intracranial Environment PoRTS Window Optical Clarity Bone Regrowth Inhibition Light Scattering Reduction Inflammation Reduction Two-photon Laser Scanning Microscopy Cerebral Blood Flow Cellular Function Anesthetized Preparations Awake Preparations Optogenetics Blood Flow Disruption

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