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解剖成年小鼠的椭圆囊和腺病毒介导的支撑细胞的感染

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection

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解剖成年小鼠的椭圆囊和腺病毒介导的支撑细胞的感染

DOI:

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11:13 min

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March 28, 2012

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Carlene S. Brandon, Christina Voelkel-Johnson, Lindsey A. May, Lisa L. Cunningham

¹Department of Pathology and Laboratory Medicine,Medical University of South Carolina, ²Department of Microbiology & Immunology,Medical University of South Carolina, ³National Institute on Deafness and Other Communication Disorders,National Institutes of Health

Capítulos

00:05Título
02:51Utricle Dissection and Culture
06:26Adenovirus Infection of Supporting Cells
07:39Utricle Fixation, Otoconia Removal, and Immunochemistry
09:06Results: Adenovirus-mediated Infection of Supporting Cells
10:19Conclusion

Summary

Tadução automática

mechanosensory毛细胞是内耳的受体细胞。最好的特点<em>在体外</em成熟的哺乳动物毛细胞模型系统利用从成年鼠的椭圆囊器官培养。我们目前的成年小鼠的囊的剥离，我们证明腺病毒介导的支持细胞在体外培养椭圆囊的感染。

Tags

Dissection Adult Mouse Utricle Adenovirus-mediated Infection Hair Cells Inner Ear Primary Organ Cultures Utricles Vestibular Organ Organ Of Corti Molecular Signals Survival Homeostasis Death Cochlear Hair Cells Regeneration Glia-like Supporting Cells Basolateral Surfaces Sensory Epithelium Adenovirus Type 5 (dE1/E3)

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