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Neuroscience

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JoVE Journal Neuroscience
Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection
 

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection

Article DOI: 10.3791/3734-v • 11:14 min • March 28th, 2012
March 28th, 2012 •
1, 2, 3, 3
1Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 2Department of Microbiology & Immunology, Medical University of South Carolina, 3National Institute on Deafness and Other Communication Disorders, National Institutes of Health

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Summary
Automatic Translation

Mechanosensory hair cells are the receptor cells of the inner ear. The best-characterized in vitro model system of mature mammalian hair cells utilizes organ cultures of utricles from adult mice. We present the dissection of the adult mouse utricle, and we demonstrate adenovirus-mediated infection of supporting cells in cultured utricles.

Tags

Dissection Adult Mouse Utricle Adenovirus-mediated Infection Hair Cells Inner Ear Primary Organ Cultures Utricles Vestibular Organ Organ Of Corti Molecular Signals Survival Homeostasis Death Cochlear Hair Cells Regeneration Glia-like Supporting Cells Basolateral Surfaces Sensory Epithelium Adenovirus Type 5 (dE1/E3)
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