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Capítulos
- 00:05Título
- 01:13Mouse Holder Assembly
- 01:58Mouse Preparation
- 03:27Surgery
- 04:552-Photon Imaging Acquisition
- 05:55Migration Analysis of GFP+ Lymphocytes in the Popliteal Lymph Node
- 08:15Conclusion
Nylige fremskridt inden for to-foton-mikroskopi har muliggjort realtid<em> I</em> Situ billeddannelse af levende væv i dyremodeller, hvorved man forbedrer evnen til at undersøge cellulær adfærd i både fysiologiske og patologiske betingelser. Her vil vi redegøre for de nødvendige forberedelser til at udføre intravital billeddannelse af musen popliteale lymfeknude.
Tags
Intravital ImagingMousePopliteal Lymph NodeLymph NodesImmune ResponseImmune Cell InteractionsLymphocytesT CellsB CellsNK CellsDendritic CellsMacrophagesBone Marrow-derived Cellular ElementsSelf AntigensForeign AntigensAntigen ReceptorsAdhesion MoleculesChemokinesStromal StructuresFibro-reticular NetworkHigh-resolution Real-time Fluorescent In Vivo ImagingStatic ImagingMorphologyPosiçãoArchitectureLymphocyte TraffickingDynamic Cell BehaviorIntravital Two-photon Laser Scanning
