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电检测屏幕的配对Nanoinjection和生物活性化合物的使用果蝇巨人纤维系统
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Neurociência
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JoVE Journal Neurociência
Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
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电检测屏幕的配对Nanoinjection和生物活性化合物的使用果蝇巨人纤维系统

DOI:
10.3791/3597-v

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09:39 min

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April 15, 2012

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, , , ,
1Department of Biological Sciences,Florida Atlantic University, 2Department of Chemistry & Biochemistry,Florida Atlantic University

Capítulos

  • 00:02Título
  • 00:48Electrophysiology rig set-up
  • 01:18Nanoinjection set-up
  • 03:14Drosophila melanogaster preparation
  • 03:58Paired nanoinjection/electrophysiology
  • 07:35Resultados

Summary

Tadução automática

Tadução automática

迅速<em>在体内</em>分析测试neuromodulatory使用巨人光纤系统（GFS）的化合物<em>果蝇</em>描述。 nanoinjections动物的头，随着政府飞行服务队的电生理记录可以揭示生物活​​性化合物对神经或肌肉。

Tags

NanoinjectionElectrophysiology AssayScreenBioactivityCompoundsDrosophila MelanogasterGiant Fiber SystemIn Vivo ActivityPharmacological AgentsModulatory EffectsNeuronal CircuitSmall MoleculesPeptidesMolecular TargetsGap JunctionsCholinergicNicotinic Acetylcholine ReceptorsNeuromuscular JunctionsGlutamatergicNanoliter QuantitiesIntracellular Recordings

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