/
/
Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
Kapitel
- 00:02Titel
- 00:48Electrophysiology rig set-up
- 01:18Nanoinjection set-up
- 03:14Drosophila melanogaster preparation
- 03:58Paired nanoinjection/electrophysiology
- 07:35Ergebnisse
A rapid in vivo assay to test for neuromodulatory compounds using the Giant Fiber System (GFS) of Drosophila melanogaster is described. Nanoinjections in the head of the animal along with electrophysiological recordings of the GFS can reveal bioactivity of compounds on neurons or muscles.
Tags
NanoinjectionElectrophysiology AssayScreenBioactivityCompoundsDrosophila MelanogasterGiant Fiber SystemIn Vivo ActivityPharmacological AgentsModulatory EffectsNeuronal CircuitSmall MoleculesPeptidesMolecular TargetsGap JunctionsCholinergicNicotinic Acetylcholine ReceptorsNeuromuscular JunctionsGlutamatergicNanoliter QuantitiesIntracellular Recordings
