É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Measurement of γHV68 Infection in Mice
13,459 Views
•
13:22 min
•
November 22, 2011
DOI:
Summary
Automatically generated
γ-Herpesviruses (γ-HVs) establish life-long persistency in their host. Infection of mice with γ-HV68 provides a genetically tractable in vivo model for the characterization of the lifecycle/pathogenesis of γHVs. This protocol describes the detection and quantitation of γHV68 infection at acute and latent stages following infection by plaque-forming, infectious center, and qPCR assays.
Explore More Videos
MeasurementγHV68InfectionMiceγ-herpesvirusesLatent InfectionsLymphoid CellsHost RangeEBVKSHVPathogenic StudiesMurine γ-herpesvirus 68Genetic SimilaritiesBiological SimilaritiesViral InfectionViral LifecyclePathogenesisIntranasal InoculationAcute ViremiaLatent InfectionSplenocytesReactivationPlaque AssayInfectious Virus TiterLung HomogenatesVero Cell MonolayersPost-intranasal InfectionAcute InfectionLatent Infection Spleen
Read Article
Cite this Article
Pirooz, S. D., Lee, J., Zhao, Z., Ni, D., Oh, S., Liang, C. Measurement of γHV68 Infection in Mice. J. Vis. Exp. (57), e3472, doi:10.3791/3472 (2011).
Copy