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Immunology and Infection

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JoVE Journal Immunology and Infection
Measurement of γHV68 Infection in Mice
 

Measurement of γHV68 Infection in Mice

Article DOI: 10.3791/3472-v • 13:23 min • November 22nd, 2011
November 22nd, 2011 •
1, 1, 1, 1, 1, 1
1Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles

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Resumen
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γ-Herpesviruses (γ-HVs) establish life-long persistency in their host. Infection of mice with γ-HV68 provides a genetically tractable in vivo model for the characterization of the lifecycle/pathogenesis of γHVs. This protocol describes the detection and quantitation of γHV68 infection at acute and latent stages following infection by plaque-forming, infectious center, and qPCR assays.

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Measurement γHV68 Infection Mice γ-herpesviruses Latent Infections Lymphoid Cells Host Range EBV KSHV Pathogenic Studies Murine γ-herpesvirus 68 Genetic Similarities Biological Similarities Viral Infection Viral Lifecycle Pathogenesis Intranasal Inoculation Acute Viremia Latent Infection Splenocytes Reactivation Plaque Assay Infectious Virus Titer Lung Homogenates Vero Cell Monolayers Post-intranasal Infection Acute Infection Latent Infection Spleen
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