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Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses
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Neurociência
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JoVE Journal Neurociência
Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses

Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses

DOI:
10.3791/3424-v

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08:38 min

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March 14, 2012

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,
1Department of Ophthalmology and Visual Sciences,Washington University School of Medicine

Capítulos

  • 00:00Visão Geral
  • 01:25Preparing the Recording Equipment
  • 04:25Isolating the Mouse Retina
  • 06:54Transretinal Recordings
  • 08:16Conclusion

Summary

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We describe a relatively simple method of transretinal electroretinogram (ERG) recordings for obtaining rod and cone photoresponses from intact mouse retina. This approach takes advantage of the block of synaptic transmission from photoreceptors to isolate their light responses and record them using field electrodes placed across the isolated flat-mounted retina.

Tags

Transretinal ERG RecordingsMouse RetinaRodsConesPhotoreceptorsVisual PigmentsPhotoresponseSuction Electrode Recording TechniqueTransretinal Electroretinogram RecordingsPharmacologically Blocked Postsynaptic Response ComponentsMouse PhotoreceptorsDark AdaptationCone Photosensitivity Recovery

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