Journal
/
Neurociencias
/
Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses

Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses

DOI:
10.3791/3424-v

•

08:38 min

•

March 14, 2012

•

,
1Department of Ophthalmology and Visual Sciences,Washington University School of Medicine

Capítulos

  • 00:00Descripción
  • 01:25Preparing the Recording Equipment
  • 04:25Isolating the Mouse Retina
  • 06:54Transretinal Recordings
  • 08:16Conclusion

Summary

Traducción Automática

Traducción Automática

We describe a relatively simple method of transretinal electroretinogram (ERG) recordings for obtaining rod and cone photoresponses from intact mouse retina. This approach takes advantage of the block of synaptic transmission from photoreceptors to isolate their light responses and record them using field electrodes placed across the isolated flat-mounted retina.

Tags

Transretinal ERG RecordingsMouse RetinaRodsConesPhotoreceptorsVisual PigmentsPhotoresponseSuction Electrode Recording TechniqueTransretinal Electroretinogram RecordingsPharmacologically Blocked Postsynaptic Response ComponentsMouse PhotoreceptorsDark AdaptationCone Photosensitivity Recovery

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Patch Clamp Recordings from Mouse Retinal Neurons in a Dark-adapted Slice Preparation

In vivo Electroporation of Developing Mouse Retina

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks

Noninvasive EEG Recordings from Freely Moving Piglets

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions

In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility

Read Article