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Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas
JoVE Journal
Medicina
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JoVE Journal Medicina
Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas

Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas

DOI:
10.3791/3403-v

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09:46 min

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November 06, 2011

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, , ,
1Neuro-Oncology Research,Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center, 2Neurosurgery Research Laboratory,Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center

Capítulos

  • 00:05Título
  • 01:32Intracranial Implantation
  • 03:35In Vivo Bioluminescence Imaging
  • 05:243D Imaging
  • 06:18Data Analysis
  • 07:17Representative In Vivo Bioluminescent Imaging Results
  • 09:06Conclusion

Summary

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Intracranial implantation of GL261 cells into C57BL/6 mice produces malignant gliomas that recapitulate many of the hallmarks of human glioblastoma multiforme. We used GL261 cells stably expressing luciferase to allow us to use in vivo imaging to follow tumor progression. The surgery and 3D in vivo imaging are demonstrated.

Tags

Intracranial Implantation3D In Vivo Bioluminescent ImagingMurine GliomasGL261Glioblastoma MultiformeIntracranial Mouse Tumor ModelFirefly LuciferaseGL261-lucGL261-luc2C57BL/6-cBrd/cBrd/Cr MiceAlbino VariantIn Vivo ImagingIVIS SpectrumCaliper Life SciencesHistological FeaturesTumor NecrosisPseudopalisadesNeovascularizationInvasionHypercellularityInflammation

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