This content is Free Access.
Capítulos
- 00:05Título
- 01:32Intracranial Implantation
- 03:35In Vivo Bioluminescence Imaging
- 05:243D Imaging
- 06:18Data Analysis
- 07:17Representative In Vivo Bioluminescent Imaging Results
- 09:06Conclusion
Intracranial implantation of GL261 cells into C57BL/6 mice produces malignant gliomas that recapitulate many of the hallmarks of human glioblastoma multiforme. We used GL261 cells stably expressing luciferase to allow us to use in vivo imaging to follow tumor progression. The surgery and 3D in vivo imaging are demonstrated.
Tags
Intracranial Implantation3D In Vivo Bioluminescent ImagingMurine GliomasGL261Glioblastoma MultiformeIntracranial Mouse Tumor ModelFirefly LuciferaseGL261-lucGL261-luc2C57BL/6-cBrd/cBrd/Cr MiceAlbino VariantIn Vivo ImagingIVIS SpectrumCaliper Life SciencesHistological FeaturesTumor NecrosisPseudopalisadesNeovascularizationInvasionHypercellularityInflammation
