Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in <em>C. elegans</em>

Wes Williams; Paola Nix; Michael Bastiani

doi:10.3791/3331

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Bioengenharia

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Construção de um baixo orçamento Laser Sistema axotomia para estudar a regeneração do axônio em C. elegans</em

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans

Construção de um baixo orçamento Laser Sistema axotomia para estudar a regeneração do axônio em C. elegans</em

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10:05 min

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November 15, 2011

DOI:

10.3791/3331-v

DOI:

10.3791/3331-v

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10:05 min

November 15, 2011

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Wes Williams, Paola Nix, Michael Bastiani

¹Department of Biology,University of Utah

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Low-budget Laser Axotomy System Study Axon Regeneration C. Elegans Assay Cost Technical Expertise Laser Ablation System Solid-state Pulse Lasers Transparent Preparations Construction Alignment Optical Path Focused Condenser Intermediate Module Dichroic Imaging Laser Beam Microscope Condenser Lens Objective Lens Final Alignment Testing

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Williams, W., Nix, P., Bastiani, M. Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans. J. Vis. Exp. (57), e3331, doi:10.3791/3331 (2011).

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