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大肠癌细胞表面蛋白的分析，使用一种抗体的芯片和荧光多路复用
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15:17 min
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September 25, 2011
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我们描述了一个过程分解大肠直肠癌（CRC）来产生可行的单个细胞，然后在定制的抗体，认识表面抗原（DotScan CRC芯片）芯片捕获。绑定到芯片的细胞亚群可以分析使用荧光标记的单克隆抗体与荧光染料的复用。
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Colorectal CancerCell Surface Protein ProfilingAntibody MicroarrayFluorescence MultiplexingCRC PrognosisProtein ExpressionPost-translational ModificationCluster Of Differentiation AntigensBiomarkersImmunohistochemistry (IHC)Cancer Sub-classificationPrognosticationMarkerClinico-pathological StagingProteomic TechniquesITRAQDiagnostic Laboratories
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Zhou, J., Belov, L., Solomon, M. J., Chan, C., Clarke, S. J., Christopherson, R. I. Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing. J. Vis. Exp. (55), e3322, doi:10.3791/3322 (2011).
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