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分离单链DNA，双链DNA和RNA病毒使用羟基磷灰石色谱从环境社区

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

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分离单链DNA，双链DNA和RNA病毒使用羟基磷灰石色谱从环境社区

DOI:

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13:46 min

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September 29, 2011

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Douglas W. Fadrosh, Cynthia Andrews-Pfannkoch, Shannon J. Williamson

¹Department of Microbial and Environmental Genomics,The J. Craig Venter Institute, ²Department of Synthetic Biology and Bioenergy,The J. Craig Venter Institute

Capítulos

00:05Título
02:35Preparation of Solutions
03:15Preparation of Econo-Column
05:34Hydroxyapatite Chromatography
09:16Desalting Nucleic Acid Samples
11:03Examples of Separation of Single-stranded DNA, Double-stranded DNA and RNA
13:07Conclusion

Summary

Tadução automática

我们描述了独立于环境病毒社区的单链DNA，双链DNA和RNA分子的有效方法。核酸是分次使用含有磷酸盐缓冲液浓度的增加羟基磷灰石色谱。这种方法允许所有病毒从环境样品中的核酸类型的隔离。

Tags

Single-stranded DNA Double-stranded DNA RNA Environmental Viral Community Hydroxyapatite Chromatography Bacteriophages Genetic Diversity Metagenomic Techniques Taxonomic Diversity Functional Potential Library Construction Protocols Nuclease Treatment Collective Gene Complement Virus Diversity Purified Nucleic Acid Subtypes Calcium Phosphate

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