/
/
Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
31,243 Views
•
13:46 min
•
September 29, 2011
DOI:
Summary
Automatically generated
We describe an efficient method to separate single-stranded DNA, double-stranded DNA and RNA molecules from environmental viral communities. Nucleic acids are fractionated using hydroxyapatite chromatography with increasing concentrations of phosphate-containing buffers. This method permits the isolation of all viral nucleic acid types from environmental samples.
Explore More Videos
Single-stranded DNADouble-stranded DNARNAEnvironmental Viral CommunityHydroxyapatite ChromatographyBacteriophagesGenetic DiversityMetagenomic TechniquesTaxonomic DiversityFunctional PotentialLibrary Construction ProtocolsNuclease TreatmentCollective Gene ComplementVirus DiversityPurified Nucleic Acid SubtypesCalcium Phosphate
Read Article
Cite this Article
Fadrosh, D. W., Andrews-Pfannkoch, C., Williamson, S. J. Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography. J. Vis. Exp. (55), e3146, doi:10.3791/3146 (2011).
Copy