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Immunology and Infection

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JoVE Journal Immunology and Infection
Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
 

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

Article DOI: 10.3791/3146-v • 13:47 min • September 29th, 2011
September 29th, 2011 •
1, 2, 1
1Department of Microbial and Environmental Genomics, The J. Craig Venter Institute, 2Department of Synthetic Biology and Bioenergy, The J. Craig Venter Institute

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We describe an efficient method to separate single-stranded DNA, double-stranded DNA and RNA molecules from environmental viral communities. Nucleic acids are fractionated using hydroxyapatite chromatography with increasing concentrations of phosphate-containing buffers. This method permits the isolation of all viral nucleic acid types from environmental samples.

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Single-stranded DNA Double-stranded DNA RNA Environmental Viral Community Hydroxyapatite Chromatography Bacteriophages Genetic Diversity Metagenomic Techniques Taxonomic Diversity Functional Potential Library Construction Protocols Nuclease Treatment Collective Gene Complement Virus Diversity Purified Nucleic Acid Subtypes Calcium Phosphate
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