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解剖宿主 - 病毒相互作用模型疱疹病毒裂解性复制
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11:28 min
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October 07, 2011
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Summary
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我们描述了一个协议来识别主机的信号分子裂解性复制的模型疱疹病毒，γ疱疹病毒68（γHV68）的关键角色。利用转基因小鼠品系和胚胎成纤维细胞裂解性复制γHV68，该协议允许表型特征和在病毒裂解复制的病毒 - 宿主相互作用的分子审问。
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Host-virus InteractionLytic ReplicationModel HerpesvirusViral InfectionInnate Immune Signaling PathwaysAntiviral Cytokine ProductionViral EvasionSignaling Pathways ManipulationTherapeutic StrategiesMurine γHV68Kaposi’s Sarcoma-associated HerpesvirusEpsten-Barr VirusSmall Animal ModelPhenotypic CharacterizationMolecular DissectionHost Signaling ComponentsGenetically Modified Mouse StrainsAcute InfectionMouse Embryonic Fibroblasts (MEFs)Virological AssaysMolecular Biology Assays
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Dong, X., Feng, P. Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus. J. Vis. Exp. (56), e3140, doi:10.3791/3140 (2011).
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