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Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
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October 07, 2011
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We describe a protocol to identify key roles of host signaling molecules in lytic replication of a model herpesvirus, gamma herpesvirus 68 (γHV68). Utilizing genetically modified mouse strains and embryonic fibroblasts for γHV68 lytic replication, the protocol permits both phenotypic characterization and molecular interrogation of virus-host interactions in viral lytic replication.
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Host-virus InteractionLytic ReplicationModel HerpesvirusViral InfectionInnate Immune Signaling PathwaysAntiviral Cytokine ProductionViral EvasionSignaling Pathways ManipulationTherapeutic StrategiesMurine γHV68Kaposi’s Sarcoma-associated HerpesvirusEpsten-Barr VirusSmall Animal ModelPhenotypic CharacterizationMolecular DissectionHost Signaling ComponentsGenetically Modified Mouse StrainsAcute InfectionMouse Embryonic Fibroblasts (MEFs)Virological AssaysMolecular Biology Assays
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Dong, X., Feng, P. Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus. J. Vis. Exp. (56), e3140, doi:10.3791/3140 (2011).
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