/
/
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
This content is Free Access.
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
42,332 Views
•
09:30 min
•
August 15, 2011
DOI:
Summary
Automatically generated
A gene transfer method into the developing mouse brain is described by using a unique surgical method and special shape of electrodes. This unique technique allows transfection of plasmid DNA temporally and spatially, which will aid many neuroscientists in studying brain development.
Explore More Videos
MouseIn Utero ElectroporationGene TransfectionDeveloping BrainGene TransferKnock-outKnock-inSpecific CRE LineTransfection MethodCandidate GenesTransgenic AnimalsBrain AreasEmbryonic LethalityElectrodesOptic Fiber Optic Light CableDNA Solution InjectionVentriclesNeedle Type ElectrodesBrain Area Patterning
Read Article
Cite this Article
Matsui, A., Yoshida, A. C., Kubota, M., Ogawa, M., Shimogori, T. Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection. J. Vis. Exp. (54), e3024, doi:10.3791/3024 (2011).
Copy