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Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
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Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
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August 15, 2011
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A gene transfer method into the developing mouse brain is described by using a unique surgical method and special shape of electrodes. This unique technique allows transfection of plasmid DNA temporally and spatially, which will aid many neuroscientists in studying brain development.
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MouseIn Utero ElectroporationGene TransfectionDeveloping BrainGene TransferKnock-outKnock-inSpecific CRE LineTransfection MethodCandidate GenesTransgenic AnimalsBrain AreasEmbryonic LethalityElectrodesOptic Fiber Optic Light CableDNA Solution InjectionVentriclesNeedle Type ElectrodesBrain Area Patterning
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Matsui, A., Yoshida, A. C., Kubota, M., Ogawa, M., Shimogori, T. Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection. J. Vis. Exp. (54), e3024, doi:10.3791/3024 (2011).
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