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Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
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神経科学
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JoVE Journal 神経科学
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

DOI:
10.3791/3024-v

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09:30 min

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August 15, 2011

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, , , ,
1Lab for Molecular Mechanisms of Thalamus Development,RIKEN Brain Science Institute

章

  • 00:05標題
  • 02:08Capillary and Micro-Electrode Preparation
  • 03:48Plasmid DNA Preparation
  • 04:47Surgery, Injection and Electroporation
  • 07:03In utero Cortical and Deep-tissue Plasmid Electroporation
  • 08:34Conclusion

概要

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自動翻訳

A gene transfer method into the developing mouse brain is described by using a unique surgical method and special shape of electrodes. This unique technique allows transfection of plasmid DNA temporally and spatially, which will aid many neuroscientists in studying brain development.

タグ

MouseIn Utero ElectroporationGene TransfectionDeveloping BrainGene TransferKnock-outKnock-inSpecific CRE LineTransfection MethodCandidate GenesTransgenic AnimalsBrain AreasEmbryonic LethalityElectrodesOptic Fiber Optic Light CableDNA Solution InjectionVentriclesNeedle Type ElectrodesBrain Area Patterning

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