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在活细胞中的荧光寿命成像分子转子
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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在活细胞中的荧光寿命成像分子转子

DOI:
10.3791/2925-v

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09:45 min

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February 09, 2012

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, , , ,
1Department of Physics,King’s College London, 2Department of Chemistry,Imperial College London , 3PhotoBiotics Ltd

Capítulos

  • 00:05Título
  • 01:43Staining Cells with Molecular Rotors
  • 02:57Fluorescence Lifetime Imaging Microscopy (FLIM) of Fluorescent Molecular Rotors in Cells
  • 04:50FLIM Data Analysis
  • 07:59Representative FLIM Results
  • 09:27Conclusion

Summary

Tadução automática

Tadução automática

荧光寿命成像（FLIM的）已经成为一项关键技术，对图像的环境和特定蛋白质在活细胞染料的相互作用。 FLIM的荧光分子转子允许映射在活细胞中的粘度。

Tags

Fluorescence Lifetime ImagingMolecular RotorsLiving CellsDiffusionChemical ReactionsBiological ProcessesIntracellular EventsViscosityDiseaseMalfunctionCellular LevelBulk ViscosityMicroviscosityFluorescence TechniquesOptical TechniquesNon-destructiveFluorescent Molecular RotorsFluorescence LifetimesQuantum YieldsViscosity Maps

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