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Journal / Neuroscience / 量化的活动顺调控元件外植体电穿孔
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JoVE Journal Neuroscience
量化的活动顺调控元件外植体电穿孔
 
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量化的活动顺调控元件外植体电穿孔

Article DOI: 10.3791/2821-v • 07:39 min • June 28th, 2011
June 28th, 2011 •
1, 1, 1
1Department of Pathology and Immunology, Washington University School of Medicine

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本协议描述了一个简单而廉价的方式来量化的顺式调控元件的活动（即，增强/促销员）通过外植体电小鼠视网膜生活。 DNA的制备，视网膜剥离，电，视网膜外植体文化，和固定后的分析和定量描述。

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神经科学，第52期，视网膜感光细胞，顺式调控元件，量化，电，启动子分析
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