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Visualizzazione delle cellule interstiziali di Cajal (ICC) di rete in topi
1Department of Medicine,Memorial Sloan Kettering Cancer Center, 2Human Oncology and Pathogenesis Program,Memorial Sloan Kettering Cancer Center, 3Developmental Biology Program,Memorial Sloan Kettering Cancer Center, 4Howard Hughes,Medical Institute, 5Laboratory of Chromatin Biology and Epigenetics,The Rockefeller University
Capítulos
- 00:05Título
- 01:35Dissection of the Mouse Gastrointestinal Tract
- 02:56Whole Mount Sample Preparation and Immunostaining
- 05:10Cryosection Preparation and Immunostaining
- 07:44Representative Imaging Results
- 09:23Conclusion
Le cellule interstiziali di Cajal (ICC) sono le cellule pacemaker del tratto gastrointestinale (GI). Essi formano reti complesse tra le cellule muscolari lisce e fibre neuronali post-gangliari per regolare contrattilità gastrointestinale. Qui vi presentiamo metodi di immunofluorescenza trasversale e con tutto il supporto di visualizzazione murini reti ICC.
Tags
VisualizationInterstitial Cells Of Cajal (ICC)MiceGastrointestinal (GI) TractPacemaker CellsSlow WavesPeristalsisEnteric Nervous SystemMotility DisordersKIT Receptor Tyrosine KinaseKIT ImmunostainingICC NetworkKIT ActivityNeoplastic TransformationGastrointestinal Stromal Tumor (GIST)ETV1 Survival FactorICC/GIST LineageTumorigenesisActivating KIT MutationsVisualization MethodsPreviously Published ProtocolsAnoctamin 1 (ANO1)Membrane MarkerImmunofluorescence
